Plasmid

Part:BBa_K4585011:Design

Designed by: Siyao Ma   Group: iGEM23_CSU-CHINA   (2023-10-04)


pcDNA3.1(+)-GnRH-C


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal SpeI site found at 249
    Illegal PstI site found at 2330
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 895
    Illegal SpeI site found at 249
    Illegal PstI site found at 2330
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 12
    Illegal XhoI site found at 1000
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal SpeI site found at 249
    Illegal PstI site found at 2330
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal SpeI site found at 249
    Illegal PstI site found at 2330
    Illegal NgoMIV site found at 1440
    Illegal NgoMIV site found at 2781
    Illegal NgoMIV site found at 3064
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 882
    Illegal BsaI.rc site found at 4589
    Illegal SapI site found at 3509
    Illegal SapI.rc site found at 2630
    Illegal SapI.rc site found at 2840


Design Notes

After sequencing and ensuring the sequence was correct, we applied it to the experiments. Store at 4℃.


Source

We designed the pcDNA3.1(+)-GnRH-C plasmid, which was obtained by inserting a GnRH-C fragment (BBa _ K4585000) into the pcDNA3.1(+) plasmid.

References